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ZWD attenuates renal water reabsorption via suppressing AQPs in NS. (A-B) Location of <t>AQP1,</t> AQP2, and AQP3 in cortex (A) and medulla (B), detected by IHC. (C) Protein levels of AQP1, AQP2, and AQP3, detected by WB ( n = 6). (D-F) mRNA levels of AQP1, AQP2, and AQP3 detected by RT-qPCR ( n = 6). Model group vs. control group (** p < 0.01, ANOVA followed by LSD); ZWD and prednisone groups vs. model group (# p < 0.05, ## p < 0.01, ANOVA followed by LSD). AQP: aquaporin; WB: western blot; ANOVA: analysis of variance.
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ZWD attenuates renal water reabsorption via suppressing AQPs in NS. (A-B) Location of <t>AQP1,</t> AQP2, and AQP3 in cortex (A) and medulla (B), detected by IHC. (C) Protein levels of AQP1, AQP2, and AQP3, detected by WB ( n = 6). (D-F) mRNA levels of AQP1, AQP2, and AQP3 detected by RT-qPCR ( n = 6). Model group vs. control group (** p < 0.01, ANOVA followed by LSD); ZWD and prednisone groups vs. model group (# p < 0.05, ## p < 0.01, ANOVA followed by LSD). AQP: aquaporin; WB: western blot; ANOVA: analysis of variance.
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ZWD attenuates renal water reabsorption via suppressing AQPs in NS. (A-B) Location of <t>AQP1,</t> AQP2, and AQP3 in cortex (A) and medulla (B), detected by IHC. (C) Protein levels of AQP1, AQP2, and AQP3, detected by WB ( n = 6). (D-F) mRNA levels of AQP1, AQP2, and AQP3 detected by RT-qPCR ( n = 6). Model group vs. control group (** p < 0.01, ANOVA followed by LSD); ZWD and prednisone groups vs. model group (# p < 0.05, ## p < 0.01, ANOVA followed by LSD). AQP: aquaporin; WB: western blot; ANOVA: analysis of variance.
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Average 96 stars, based on 1 article reviews
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ZWD attenuates renal water reabsorption via suppressing AQPs in NS. (A-B) Location of AQP1, AQP2, and AQP3 in cortex (A) and medulla (B), detected by IHC. (C) Protein levels of AQP1, AQP2, and AQP3, detected by WB ( n = 6). (D-F) mRNA levels of AQP1, AQP2, and AQP3 detected by RT-qPCR ( n = 6). Model group vs. control group (** p < 0.01, ANOVA followed by LSD); ZWD and prednisone groups vs. model group (# p < 0.05, ## p < 0.01, ANOVA followed by LSD). AQP: aquaporin; WB: western blot; ANOVA: analysis of variance.

Journal: Renal Failure

Article Title: Zhenwu decoction ameliorates adriamycin-induced nephrotic syndrome in rats via dual modulation of AVP-V2R-AQP2 and RAAS-MR-AQP3 pathways

doi: 10.1080/0886022X.2025.2599570

Figure Lengend Snippet: ZWD attenuates renal water reabsorption via suppressing AQPs in NS. (A-B) Location of AQP1, AQP2, and AQP3 in cortex (A) and medulla (B), detected by IHC. (C) Protein levels of AQP1, AQP2, and AQP3, detected by WB ( n = 6). (D-F) mRNA levels of AQP1, AQP2, and AQP3 detected by RT-qPCR ( n = 6). Model group vs. control group (** p < 0.01, ANOVA followed by LSD); ZWD and prednisone groups vs. model group (# p < 0.05, ## p < 0.01, ANOVA followed by LSD). AQP: aquaporin; WB: western blot; ANOVA: analysis of variance.

Article Snippet: The primary antibodies used were against MR (diluted 1:500, BS-1850R, BIOSS), V2R (diluted 1:1000, BS-10014R, BIOSS), AQP1 (diluted 1:1000, AF5231, Affinity), AQP2 (diluted 1:2000, DF7560, Affinity), AQP3 (diluted 1:2000, BS-1253R, BIOSS), and β-actin (diluted 1:30000, 66009-1-Ig, Proteintech, USA).

Techniques: Quantitative RT-PCR, Control, Western Blot

Compound–major target molecular docking analysis. (A) Initial 3D conformation of unoptimized bioactive compounds. (B) 3D conformation of optimized bioactive compounds. (C) Interaction mode of AQP1 with benzoylpaeoniflorin. (D–F) Interaction mode of AQP2, V2R, and MR with 6-gingerol. (G) Interaction mode of AQP3 with paeoniflorin. AQP: aquaporin; V2R: type 2 vasopressin receptor; MR: mineralocorticoid receptor.

Journal: Renal Failure

Article Title: Zhenwu decoction ameliorates adriamycin-induced nephrotic syndrome in rats via dual modulation of AVP-V2R-AQP2 and RAAS-MR-AQP3 pathways

doi: 10.1080/0886022X.2025.2599570

Figure Lengend Snippet: Compound–major target molecular docking analysis. (A) Initial 3D conformation of unoptimized bioactive compounds. (B) 3D conformation of optimized bioactive compounds. (C) Interaction mode of AQP1 with benzoylpaeoniflorin. (D–F) Interaction mode of AQP2, V2R, and MR with 6-gingerol. (G) Interaction mode of AQP3 with paeoniflorin. AQP: aquaporin; V2R: type 2 vasopressin receptor; MR: mineralocorticoid receptor.

Article Snippet: The primary antibodies used were against MR (diluted 1:500, BS-1850R, BIOSS), V2R (diluted 1:1000, BS-10014R, BIOSS), AQP1 (diluted 1:1000, AF5231, Affinity), AQP2 (diluted 1:2000, DF7560, Affinity), AQP3 (diluted 1:2000, BS-1253R, BIOSS), and β-actin (diluted 1:30000, 66009-1-Ig, Proteintech, USA).

Techniques: